Ingredient innovation


Native and emulsified milk fat globule size is considered a pivotal processing and functionality parameter in many fat-structured dairy products. This project aims to manipulate different sized milk fat globules (MFG) to achieve better physical functionality of fat components in selected high-fat dairy products, eg. cream, butter and spreads (Figure 1). This includes both fundamental and application studies along with pilot scale trials, aiming to provide controllable and scalable techniques that can be translated to the dairy industry.

Figure 1. Milk fat crystallisation. (a) In bulk, using polarised light microscopy, (b) in nanoemulsion droplets, using cryo transmission electron microscopy (cryo TEM) and (c) in butter, using confocal laser scanning microscopy (CLSM) (fat (green), solid fat (dark area) and water (red).

A technique, known as microfluidization, has been used to reduce MFGs to submicron- and nano-sized proportions. A two-stage separation method has been developed to fractionate native milk and cream on the basis of fat globule size. These size differentiated milk streams and creams will be used to manufacture butter, fat-reduced dairy spreads, low-fat cream and cream powders. An innovative approach using dissolved CO2 in milk fat to modify milk fat crystallisation has also been evaluated and applied to butter making. The findings on different sized MFGs will assist the development of innovative dairy products. A two-stage separation method and the utilisation of CO2-enhanced crystallisation can be used as additional tools to control or modify manufacturing processes (Figure 2).

Figure 2. Overview of research activities.

Capabilities and technologies


Formation of small droplets at the submicron- to nano-scale requires large amounts of surfactant and/or mechanical energy (microfluidization). Microfluidization is a process that utilises intensively disruptive forces created by the collision of two rapidly flowing streams in an interaction chamber. Nano-sized creams (< 200 nm) have been prepared using this method.

Two-stage fractionation of dairy cream

A novel two-stage separation method has been developed to fractionate milk/cream on the basis of mean MFG size. The first stage involves a unique centrifugal separation technique, where two streams of milk, one rich in large fat globules and another rich in small fat globules, are obtained by fractionation in a modified cream separator. In the second stage, the two streams from the first stage are each further fractionated in a conventional cream separator.

CO2-enhanced crystallisation

The crystallisation of solid foods (fats/oils, lactose, water) can be influenced by dissolving or non-dissolving entities present in the food matrix. Carbon dioxide is a hydrophobic gas that is soluble in lipids and water. This gas is used in the modified atmospheric packaging of fruits, vegetables, drinks, meat and cheese. The gas is non-toxic and environmentally safe. Being a soluble gas in a food system, it can take part in the crystallisation process, in turn, affecting product properties. We have developed methods to dissolve CO2 into anhydrous milk fat (AMF) at various scales (Figure 3).

Figure 3. Infusion of CO2 into milk fat and/or edible lipids can be achieved at various scales. CO2 can be dissolved in molten milk fat at the lab-scale (partial pressure < 1 bar) using (a) a bottle (600 mL) or (b) a beer keg (10 L). Changes of CO2 partial pressures are monitored by the attached manometer. (c) Pilot-scale trial performed by CI Bhesh Bhandari using a continuous CO2 infusion system (50 L) at DIAL.

Characterisation techniques

Our core analysis capabilities include differential scanning calorimetry (DSC), small angle X-ray scattering/wide angle X-ray scattering (SAXS/WAXS), texture analysis, electron microscopy, confocal laser scanning microscopy (CLSM), rheology, tribology, and solid-phase microextraction gas chromatography-mass spectrometry (SPME GC-MS).  The facilities support both the Ingredient Innovation team and other Hub members through collaborative research arrangements and can also be accessed as fee-for-service.


Butter has been manufactured from nano-sized cream (< 200 nm) using a temperature-controlled Stephan mixer (Figure 4).

Figure 4. MFGs in dairy cream (40% fat) can be reduced to nano-sized MFGs (< 200 nm) using a microfluidizer. This cream can then be processed into butter using a Stephan mixer. The butter made from nano-sized cream has different characteristics compared to that produced from unmodified dairy cream.

A two-stage separation process has been developed that can create streams with a mean fat globule size (D [4,3]) as small as 1.35 µm and as large as 4.28 µm without affecting droplet integrity (Figure 5). With further development, given its high throughput, good separation efficiency and use of conventional processing equipment, this method could provide the basis for a new industrial process to generate fractionated milk streams and creams with contrasting physical functionality and nutritional value, based on MFG size.

Figure 5. Size differentiated cream fractions obtained from the two-stage fractionation of cream.

We have demonstrated that dissolved CO2 can be used to modulate the crystallisation behaviour, microstructure and texture of anhydrous milk fat (AMF). The addition of CO2 has been shown to enhance crystallinity, accelerate crystal nucleation and induce a larger number of smaller crystals than observed in untreated AMF. When applying this technique to butter making, we found that carbonation (0–2000 ppm) of warm cream (35°C) can be used to reduce the churning time of the butter making process. Carbonation did not show any adverse effect on colour, microstructure (Figure 6) and sensory quality of the resultant butter.

Figure 6. (a) Butter made from carbonated cream (2000 ppm) and (b) CLSM image showing the microstructure as a continuous fat phase (green) with solid fat crystals (black) in which water droplets (red) and air cells are embedded.


Dhungaga, P., Truong, T., Palmer, M., Bansal, N. & Bhandari, B. (2017). Size-based fractionation of native milk fat globules by two-stage centrifugal separation. Innovative Food Science and Emerging Technologies, 41, 235–243.

Truong, T., Palmer, M., Bansal, N. & Bhandari, B. (2017). Effect of solubilized CO2 at low partial pressure on crystallisation behaviour, microstructure and texture of anhydrous milk fat. Food Research International, 95, 82–90.

Hussain, H., Truong, T., Bansal, N. & Bhandari, B. (2017). The effect of manipulating fat globule size on the stability and rheological properties of dairy creams. Food Biophysics, 12(1), 1–10.

Truong, T., Palmer, M., Bansal, N. & Bhandari, B. (2016). Effect of Milk Fat Globule Size on the Physical Functionality of Dairy Products. AG Switzerland: Springer International Publishing.


  • Prof Bhesh Bhandari

    Chief Investigator

  • Dr Tuyen Truong

    Postdoctoral Researcher

  • Pramash Dhungana

    PhD Student

  • Bravesh Panchal

    PhD Student